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This Concept Map, created with IHMC CmapTools, has information related to: LAB 11 - VIRUSES, Viruses are infectious agents with both living and non-living characteristics. non-living characteristics a. They are acellular, that is, they contain no cytoplasm or cellular organelles. b. They carry out no metabolism on their own and must replicate using the host cell's metabolic machinery. Viruses don't grow and divide. Instead, new viral components are synthesized and assembled within the infected host cell. c. With few exceptions, they possess DNA or RNA but never both., plaque formation step 1 Using a 1.0 ml pipette, dilute the Coliphage T4 stock as shown in Fig. 8 in your lab manual., Using a new 1.0 ml pipette, add 0.1 ml of each bacteriophage dilution to its corresponding empty sterile tube as showm in Fig. 8 in your lab manual. step 3 Using a new sterile 1.0 ml pipette, add 0.5 ml of E. coli B to the 0.1 ml of bacteriophage in each of the 3 tubes from step 2 as shown in Fig. 9 in your lab manual., Bacteriophages are viruses that infect only bacteria. terminology viral specificity, viral specificity step 1 Label 2 plates of TSA as shown in Fig. 13 in your lab manual., Bacteriophages are viruses that infect only bacteria. procedures plaque formation, replicate by the lysogenic life cycle (Do not have to know for lab.) lysogenic life cycle When a temperate bacteriophage infects a bacterium, it can either: 1) replicate by the lytic life cycle and cause lysis of the host bacterium, or it can 2) incorporate its DNA into the bacterium's DNA and assume a noninfectious state called a prophage. Expression of the bacteriophage genes controlling bacteriophage replication is repressed. Only during rare spontaneous induction does the virus replicate and assemble., Label 2 plates of TSA as shown in Fig. 13 in your lab manual. step 2 Streak unknown bacterium #1 on sector 1 of the first TSA plate by streaking the loop through the circle you drew. Streak unknown #2 on sector 2; unknown #3 on sector 3; and unknown #4 on sector 4., Viruses are infectious agents with both living and non-living characteristics. bacteriophages Bacteriophages are viruses that infect only bacteria., Bacteriophages are viruses that infect only bacteria. terminology plaques, Bacteriophages are viruses that infect only bacteria. structure A genome surrounded by a protein capsid. Some, like coliphage T4 have a complex tail structure., replicate by the lytic life cycle lytic life cycle step 1 Adsorption: Attachment sites on the bacteriophage tail adsorb to receptor sites on the cell wall of a susceptible host bacterium., Bacteriophages are viruses that infect only bacteria. temperate bacteriophage replicate by the lysogenic life cycle (Do not have to know for lab.), Quickly pour the Motility Medium-E. coli-bacteriophage mixtures onto separate plates of Trypticase Soy agar and swirl to distribute the contents over the entire agar surface. step 6 Incubate the 3 TSA plates right-side-up at 37°C until the next lab period., Bacteriophages are viruses that infect only bacteria. terminology phage typing, Using a new sterile 1.0 ml pipette, add 0.5 ml of E. coli B to the 0.1 ml of bacteriophage in each of the 3 tubes from step 2 as shown in Fig. 9 in your lab manual. step 4 Using a sterile 10.0 ml pipette, add 2.5 ml of sterile, melted Motility Test Medium to the E. coli-bacteriophage mixture in each of the 3 tubes from step 3 as shown in Fig. 10 in your lab manual., Bacteriophages are viruses that infect only bacteria. lytic bacteriophage replicate by the lytic life cycle, Have your instructor add add 1 drop of Coliphage T4 to each sector in the area outlined by the circle. step 4 Incubate the 2 TSA plates right side up at 37°C until the next lab period., plaques definition A small, clear area on an agar plate where the host bacteria have been lysed as a result of the lytic life cycle of the infecting bacteriophages., Bacteriophages are viruses that infect only bacteria. procedures viral specificity
